Two Glycine Riboswitches Activate the Glycine Cleavage System Essential for Glycine Detoxification in Streptomyces griseus
نویسندگان
چکیده
منابع مشابه
Regulation of the hepatic glycine-cleavage system.
The primary catabolic fate of glycine in mammals involves the hepatic glycine-cleavage system in which glycine is sequentially converted to CO,, NH,, NADH + H + , and NS, N"-methylenetetrahydrofolate (Fig. 1) (for review, see Kikuchi, 1973). This multi-enzyme reaction is located exclusively in the mitochondrial compartment (Motokawa 81 Kikuchi, 1971) and can be compared in several respects with...
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The glycine cleavage system (GCS) is the essential enzyme complex for degrading glycine and supplying 5,10-methylenetetrahydrofolate for DNA synthesis. Inherited deficiency of this system causes nonketotic hyperglycinemia, characterized by severe neurological symptoms and frequent association of brain malformations. Although high levels of glycine have been considered to cause the above-mention...
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A set of 12 fluorogenic glycine riboswitches with different thermodynamic and kinetic response properties was engineered. For the design of functional riboswitches, a three-part RNA approach was applied based on the idea of linking a RNA sensor, transmitter and actuator part together. For the RNA sensor and actuator part, we used the tandem glycine aptamer structure from Bacillus subtillis, and...
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stimulates glutamine release at the expense of tissue glutamate while decreasing or having little effect on alanine release (Ruderman & Lund, 1972; Chang & Goldberg, 1978). In the perfused rat hindlimb total glutamine production by resting muscle is over 4-fold greater than ammonia production, but the addition of methionine sulphoximine (a glutamine synthetase inhibitor) reverses this pattern (...
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One of the four proteins involved in the cleavage of glycine to l-carbon units and the transfer of electrons from glycine to diphosphopyridine nucleotide is a flavoprotein. Precipitation of the protein with acid ammonium sulfate in the presence of 1 M potassium bromide separated the coenzyme from the apoenzyme. The latter was fully reactivated by incubation with flak adenine dinucleotide, but s...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2014
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.01480-13